Vertically integrated optical transducer for bio-particle detection

Marianah Masrie*, Jumril Yunas, Burhanuddin Yeop Majlis, Arash Dehzangi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

An optical transducer with vertical structure integrated with MEMS microfluidic device is developed for the detection of bio-particle. The system consists of optical detection part; electronics control part and microfluidic part. Integrating of these parts, using optical transducer as a label-free method can provide the diagnosis of low volume biological samples as well as leading to direct detection with no significant change in physical forms of the samples. A collimated light source of deep UV-AlGaN based LED and a SiC photodiode operating at 260 nm are utilized as a light transmitter and detector, respectively. The principle of bio-particle detection is based on absorption of ultraviolet (UV) range of DNA biological samples extracted from a Caco-2 cell in a fluidic medium. The outcomes show that the signal is detected by the SiC photodiode for the rise time of τr ~ 118.1371 µs which shows sufficient response time to enable the detection of the bio-particle. Testing on various samples showed that the detector has no response to non-absorbance samples such as deionized (DI) water, Tris-EDTA (TE) buffer and protein samples. However, a significant outcome in the detection of a Caco-2 cell line with the absorbance coefficient of average 0.08 a.u was achieved.

Original languageEnglish (US)
Pages (from-to)1886-1899
Number of pages14
JournalJournal of Engineering Science and Technology
Volume12
Issue number7
StatePublished - Jul 2017

Keywords

  • MEMS device
  • Optical transducer
  • PDMS microfluidic
  • SiC photodiode
  • UV LED

ASJC Scopus subject areas

  • Engineering(all)

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