Vibrational imaging of tablets by epi-detected stimulated Raman scattering microscopy

Mikhail N. Slipchenko; Hongtao Chen; David R. Ely; Yookyung Jung; M. Teresa Carvajal; Ji Xin Cheng

doi:10.1039/c0an00252f

Vibrational imaging of tablets by epi-detected stimulated Raman scattering microscopy

Mikhail N. Slipchenko, Hongtao Chen, David R. Ely, Yookyung Jung, M. Teresa Carvajal, Ji Xin Cheng

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

Proper chemical imaging tools are critical to the pharmaceutical industry due to growing regulatory demand for intermediate and end-product content uniformity testing. Herein we demonstrate stimulated Raman scattering (SRS) imaging of active pharmaceutical ingredient (API) and four excipients within tablets. Tablets from six manufactures were imaged with a speed of 53 s per frame of 512 × 512 pixels (i.e., 200 μs per pixel) and a lateral spatial resolution as high as 0.62 μm. The SRS chemical imaging was compared to confocal Raman mapping and coherent anti-Stokes Raman scattering (CARS) chemical imaging in terms of speed and chemical selectivity. The acquisition speed of SRS imaging is ca. 10⁴ times faster than confocal Raman mapping and SRS technique showed superior to CARS chemical selectivity for studied samples. Our data demonstrate the potential of SRS microscopy in high-speed screening of pharmaceutical solid dosage forms.

Original language	English (US)
Pages (from-to)	2613-2619
Number of pages	7
Journal	Analyst
Volume	135
Issue number	10
DOIs	https://doi.org/10.1039/c0an00252f
State	Published - Oct 2010
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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abstract = "Proper chemical imaging tools are critical to the pharmaceutical industry due to growing regulatory demand for intermediate and end-product content uniformity testing. Herein we demonstrate stimulated Raman scattering (SRS) imaging of active pharmaceutical ingredient (API) and four excipients within tablets. Tablets from six manufactures were imaged with a speed of 53 s per frame of 512 × 512 pixels (i.e., 200 μs per pixel) and a lateral spatial resolution as high as 0.62 μm. The SRS chemical imaging was compared to confocal Raman mapping and coherent anti-Stokes Raman scattering (CARS) chemical imaging in terms of speed and chemical selectivity. The acquisition speed of SRS imaging is ca. 104 times faster than confocal Raman mapping and SRS technique showed superior to CARS chemical selectivity for studied samples. Our data demonstrate the potential of SRS microscopy in high-speed screening of pharmaceutical solid dosage forms.",

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AU - Jung, Yookyung

AU - Carvajal, M. Teresa

AU - Cheng, Ji Xin

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Vibrational imaging of tablets by epi-detected stimulated Raman scattering microscopy

Abstract

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