VIGR - A novel inducible adhesion family G-protein coupled receptor in endothelial cells

Christian Stehlik, Renate Kroismayr, Andrea Dorfleutner, Bernd R. Binder, Joachim Lipp*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Using a signal sequence trap for selection of differentially expressed secretory and membrane proteins, we identified a novel member of the adhesion family of G-protein coupled receptors (GPCRs), termed vascular inducible GPCR (VIGR). VIGR contains C1r-C1s, Uegf and Bmp1 (CUB) and pentraxin (PTX)-like modules and a mucin-like spacer, followed by seven transmembrane domains. By surface biotinylation as well as by immunofluorescence analysis we demonstrate that endogenous, highly glycosylated VIGR is expressed on the cell surface of endothelial cells (ECs) upon LPS or thrombin treatment, and inducible expression is mediated by MAP kinases, but not NF-κB. We show that VIGR is selectively expressed in ECs derived from larger vessels, but not from microvessels. In summary, VIGR represents a novel GPCR of the adhesion family, which is unique in its long extra-cellular domain comprising CUB and PTX-like modules and in its inducibility by LPS and thrombin in a subset of ECs, suggesting an important function in cell-adhesion and potentially links inflammation and coagulation.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalFEBS Letters
Volume569
Issue number1-3
DOIs
StatePublished - Jul 2 2004

Keywords

  • CUB, C1r-C1s, Uegf and Bmp1
  • EC, endothelial cell
  • GPCR, G-protein coupled receptor
  • GPS, GPCR proteolytic site
  • HAECs, human aortic ECs
  • HSMECs, human skin microvascular ECs
  • HUVECs, human umbilical vein ECs
  • LPS, lipopolysaccharide

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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