Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition

Melissa G. Mendez, Shin Ichiro Kojima, Robert D. Goldman

Research output: Contribution to journalArticlepeer-review

508 Scopus citations

Abstract

Vimentin is used widely as a marker of the epithelial to mesenchymal transitions (EMTs) that take place during embryogenesis and metastasis, yet the functional implications of the expression of this type III intermediate filament (IF) protein are poorly understood. Using form factor analysis and quantitative Western blotting of normal, metastatic, and vimentinnull cell lines, we show that the level of expression of vimentin IFs (VIFs) correlates with mesenchymal cell shape and motile behavior. The reorganization of VIFs caused by expressing a dominant-negative mutant or by silencing vimentin with shRNA (neither of which alter microtubule or microfilament assembly) causes mesenchymal cells to adopt epithelial shapes. Following the microinjection of vimentin or transfection with vimentin cDNA, epithelial cells rapidly adopt mesenchymal shapes coincident with VIF assembly. These shape transitions are accompanied by a loss of desmosomal contacts, an increase in cell motility, and a significant increase in focal adhesion dynamics. Our results demonstrate that VIFs play a predominant role in the changes in shape, adhesion, and motility that occur during the EMT.

Original languageEnglish (US)
Pages (from-to)1838-1851
Number of pages14
JournalFASEB Journal
Volume24
Issue number6
DOIs
StatePublished - Jun 2010

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition'. Together they form a unique fingerprint.

Cite this