Viral transformation of epithelial cells

Jennifer A. Regan, Laimonis A. Laimins*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Approximately 18% of human cancers have a viral etiology and the majority of these involve transformation of epithelial cells. Viral proteins transform epithelia by inducing alterations in the normal cell growth and differentiation pathways through the targeting of host proteins. Among the DNA viruses responsible for causing carcinomas are the human papillomaviruses as well as several members of the herpes and polyomavirus families. A number of techniques have been developed to study the mechanisms by which viruses immortalize epithelial cells and alter differentiation properties. These methods include the generation of immortalized lines by transfection or infection as well as the use of organotypic raft cultures, suspension in methylcellulose, and treatment with high calcium levels to examine how differentiation is altered.

Original languageEnglish (US)
Title of host publicationEpithelial Cell Culture Protocols
Subtitle of host publicationSecond Edition
EditorsScott Randell, Leslie Fulcher
Pages449-465
Number of pages17
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume945
ISSN (Print)1064-3745

Keywords

  • Differentiation
  • Epithelia
  • Human papillomavirus
  • Transformation
  • Virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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