Virulence associated with outbreak-related strains of Burkholderia cepacia complex among a cohort of patients with bacteremia

Christophar W. Woods*, Adam M. Brasslar, John J. LiPuma, Barbara D. Alexander, Dennis A. Clements, David J. Weber, Christopher M. Moore, L. Barth Reller, Keith S. Kaye

*Corresponding author for this work

Research output: Contribution to journalReview article

40 Citations (Scopus)

Abstract

The Burkholderia cepacia complex includes 9 genomovars. The relative virulence of each is unknown. Host and pathogen features associated with mortality were evaluated among patients with B. cepacia complex bacteremia. Cases were ascertained through review of blood culture results for the period of May 1996 through May 2002. Isolates were identified to species level with 16S rDNA and recA-based species-specific polimerase chain reaction analyses and recA restriction fragment-length polymorphism. Strain typing was performed with pulsed-field gel electrophoresis. Fifty-three patients with B. cepacia complex bacteremia were identified; only 9 (17%) had cystic fibrosis. Twenty-five patients (47%) died within 14 days of bacteremia. After controlling for comorbid conditions and therapeutic interventions, 2 outbreak-related strains of Burkholderia cenocepacia (genomovar III) were associated with 14-day mortality (odds ratio, 5.5; 95% confidence interval, 1.20-25.02). B. cenocepacia is an emerging nosocomial pathogen. Certain strains are associated with an enhanced capacity for interpatient spread and poor outcome.

Original languageEnglish (US)
Pages (from-to)1243-1250
Number of pages8
JournalClinical Infectious Diseases
Volume38
Issue number9
DOIs
StatePublished - May 1 2004

Fingerprint

Burkholderia cepacia complex
Burkholderia cenocepacia
Bacteremia
Disease Outbreaks
Virulence
Mortality
Pulsed Field Gel Electrophoresis
Ribosomal DNA
Cystic Fibrosis
Restriction Fragment Length Polymorphisms
Odds Ratio
Confidence Intervals
Therapeutics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Woods, C. W., Brasslar, A. M., LiPuma, J. J., Alexander, B. D., Clements, D. A., Weber, D. J., ... Kaye, K. S. (2004). Virulence associated with outbreak-related strains of Burkholderia cepacia complex among a cohort of patients with bacteremia. Clinical Infectious Diseases, 38(9), 1243-1250. https://doi.org/10.1086/383313
Woods, Christophar W. ; Brasslar, Adam M. ; LiPuma, John J. ; Alexander, Barbara D. ; Clements, Dennis A. ; Weber, David J. ; Moore, Christopher M. ; Reller, L. Barth ; Kaye, Keith S. / Virulence associated with outbreak-related strains of Burkholderia cepacia complex among a cohort of patients with bacteremia. In: Clinical Infectious Diseases. 2004 ; Vol. 38, No. 9. pp. 1243-1250.
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Woods, CW, Brasslar, AM, LiPuma, JJ, Alexander, BD, Clements, DA, Weber, DJ, Moore, CM, Reller, LB & Kaye, KS 2004, 'Virulence associated with outbreak-related strains of Burkholderia cepacia complex among a cohort of patients with bacteremia', Clinical Infectious Diseases, vol. 38, no. 9, pp. 1243-1250. https://doi.org/10.1086/383313

Virulence associated with outbreak-related strains of Burkholderia cepacia complex among a cohort of patients with bacteremia. / Woods, Christophar W.; Brasslar, Adam M.; LiPuma, John J.; Alexander, Barbara D.; Clements, Dennis A.; Weber, David J.; Moore, Christopher M.; Reller, L. Barth; Kaye, Keith S.

In: Clinical Infectious Diseases, Vol. 38, No. 9, 01.05.2004, p. 1243-1250.

Research output: Contribution to journalReview article

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AU - Woods, Christophar W.

AU - Brasslar, Adam M.

AU - LiPuma, John J.

AU - Alexander, Barbara D.

AU - Clements, Dennis A.

AU - Weber, David J.

AU - Moore, Christopher M.

AU - Reller, L. Barth

AU - Kaye, Keith S.

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Y1 - 2004/5/1

N2 - The Burkholderia cepacia complex includes 9 genomovars. The relative virulence of each is unknown. Host and pathogen features associated with mortality were evaluated among patients with B. cepacia complex bacteremia. Cases were ascertained through review of blood culture results for the period of May 1996 through May 2002. Isolates were identified to species level with 16S rDNA and recA-based species-specific polimerase chain reaction analyses and recA restriction fragment-length polymorphism. Strain typing was performed with pulsed-field gel electrophoresis. Fifty-three patients with B. cepacia complex bacteremia were identified; only 9 (17%) had cystic fibrosis. Twenty-five patients (47%) died within 14 days of bacteremia. After controlling for comorbid conditions and therapeutic interventions, 2 outbreak-related strains of Burkholderia cenocepacia (genomovar III) were associated with 14-day mortality (odds ratio, 5.5; 95% confidence interval, 1.20-25.02). B. cenocepacia is an emerging nosocomial pathogen. Certain strains are associated with an enhanced capacity for interpatient spread and poor outcome.

AB - The Burkholderia cepacia complex includes 9 genomovars. The relative virulence of each is unknown. Host and pathogen features associated with mortality were evaluated among patients with B. cepacia complex bacteremia. Cases were ascertained through review of blood culture results for the period of May 1996 through May 2002. Isolates were identified to species level with 16S rDNA and recA-based species-specific polimerase chain reaction analyses and recA restriction fragment-length polymorphism. Strain typing was performed with pulsed-field gel electrophoresis. Fifty-three patients with B. cepacia complex bacteremia were identified; only 9 (17%) had cystic fibrosis. Twenty-five patients (47%) died within 14 days of bacteremia. After controlling for comorbid conditions and therapeutic interventions, 2 outbreak-related strains of Burkholderia cenocepacia (genomovar III) were associated with 14-day mortality (odds ratio, 5.5; 95% confidence interval, 1.20-25.02). B. cenocepacia is an emerging nosocomial pathogen. Certain strains are associated with an enhanced capacity for interpatient spread and poor outcome.

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