Visualization of mouse pancreas architecture using MR microscopy

Paul J. Grippo*, Palamadai N. Venkatasubramanian, Richard H. Knop, Daniel M. Heiferman, Gheorghe Iordanescu, Laleh G. Melstrom, Kevin Adrian, Morgan R. Barron, David Jason Bentrem, Alice M. Wyrwicz

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Pancreatic diseases, which include diabetes, pancreatitis, and pancreatic cancer, are often difficult to detect and/or stage, contributing to a reduced quality of life and lifespan for patients. Thus, there is need for a technology that can visualize tissue changes in the pancreas, improve understanding of disease progression, and facilitate earlier detection in the human population. Because of low spatial resolution, current clinical magnetic resonance imaging (MRI) at low field strength has yet to fully visualize the exocrine, endocrine, vascular, and stromal components of the pancreas. We used high field strength magnetic resonance microscopy (μMRI) to image mouse pancreas ex vivo without contrast agents at high spatial resolution. We analyzed the resulting high-resolution images using volume rendering to resolve components in the pancreas, including acini, islets, blood vessels, and extracellular matrix. Locations and dimensions of pancreatic components as seen in three-dimensional μMRI were compared with histological images, and good correspondence was found. Future longitudinal studies could expand on the use of in vivo μMRI in mouse models of pancreatic diseases. Capturing three-dimensional structural changes through μMRI could help to identify early cellular and tissue changes associated with pancreatic disease, serving as a mode of improved detection in the clinic for endocrine and exocrine pathologies.

Original languageEnglish (US)
Pages (from-to)610-618
Number of pages9
JournalAmerican Journal of Pathology
Volume179
Issue number2
DOIs
StatePublished - Aug 1 2011

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Pancreatic Diseases
Pancreas
Microscopy
Magnetic Resonance Imaging
Blood Vessels
Pancreatic Neoplasms
Pancreatitis
Contrast Media
Extracellular Matrix
Longitudinal Studies
Disease Progression
Magnetic Resonance Spectroscopy
Quality of Life
Pathology
Technology
Population

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Grippo, P. J., Venkatasubramanian, P. N., Knop, R. H., Heiferman, D. M., Iordanescu, G., Melstrom, L. G., ... Wyrwicz, A. M. (2011). Visualization of mouse pancreas architecture using MR microscopy. American Journal of Pathology, 179(2), 610-618. https://doi.org/10.1016/j.ajpath.2011.04.007
Grippo, Paul J. ; Venkatasubramanian, Palamadai N. ; Knop, Richard H. ; Heiferman, Daniel M. ; Iordanescu, Gheorghe ; Melstrom, Laleh G. ; Adrian, Kevin ; Barron, Morgan R. ; Bentrem, David Jason ; Wyrwicz, Alice M. / Visualization of mouse pancreas architecture using MR microscopy. In: American Journal of Pathology. 2011 ; Vol. 179, No. 2. pp. 610-618.
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Grippo, PJ, Venkatasubramanian, PN, Knop, RH, Heiferman, DM, Iordanescu, G, Melstrom, LG, Adrian, K, Barron, MR, Bentrem, DJ & Wyrwicz, AM 2011, 'Visualization of mouse pancreas architecture using MR microscopy', American Journal of Pathology, vol. 179, no. 2, pp. 610-618. https://doi.org/10.1016/j.ajpath.2011.04.007

Visualization of mouse pancreas architecture using MR microscopy. / Grippo, Paul J.; Venkatasubramanian, Palamadai N.; Knop, Richard H.; Heiferman, Daniel M.; Iordanescu, Gheorghe; Melstrom, Laleh G.; Adrian, Kevin; Barron, Morgan R.; Bentrem, David Jason; Wyrwicz, Alice M.

In: American Journal of Pathology, Vol. 179, No. 2, 01.08.2011, p. 610-618.

Research output: Contribution to journalArticle

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Grippo PJ, Venkatasubramanian PN, Knop RH, Heiferman DM, Iordanescu G, Melstrom LG et al. Visualization of mouse pancreas architecture using MR microscopy. American Journal of Pathology. 2011 Aug 1;179(2):610-618. https://doi.org/10.1016/j.ajpath.2011.04.007