'VIT1', a novel gene associated with vitiligo

I. Caroline Le Poole*, Rangaprasad Sarangarajan, Yang Zhao, Lawrence S. Stennett, Thomas L. Brown, Pranav Sheth, Toru Miki, Raymond E. Boissy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

To define genes associated with the pigmentary disorder vitiligo, gene expression was compared in non-lesional melanocytes cultured from three vitiligo patients and from three control melanocyte cultures by differential display. A basic local alignment search tool search did not reveal homology of six differentially expressed cDNA fragments to previously identified expressed sequence tags; thus, one was used to screen a melanocyte cDNA library. The underlying VIT1 gene maps to chromosome 2p16. The 3′ portion of the VIT1 message is complementary to the 3′ end of hMSH6 mRNA, enabling the formation of RNA-RNA hybrids, which may interfere with G/T mismatch repair function. Moreover, the aligned cDNA sequence revealed an open reading frame identical to a hypothetical protein expressed in brain, with a similarity to Drosophila calmodulin, and containing a zinc-finger motif partially identical to N-recognin. Expression of ORF mRNA was confirmed for multiple skin cell types, suggesting its importance for skin physiology.

Original languageEnglish (US)
Pages (from-to)475-484
Number of pages10
JournalPigment Cell Research
Volume14
Issue number6
DOIs
StatePublished - 2001

Keywords

  • Calmodulin
  • Depigmentation
  • Differential display
  • Gene expression
  • hMSH6

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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