Voltage-clamp analysis of a transient potassium current in rat neostriatal neurons

D. James Surmeier; Jose Bargas; S. T. Kitai

doi:10.1016/0006-8993(88)90334-4

Voltage-clamp analysis of a transient potassium current in rat neostriatal neurons

D. James Surmeier^*, Jose Bargas, S. T. Kitai

^*Corresponding author for this work

Physiology

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Whole cell voltage-clamp recording were made from cultured rat neostriatal neurons. Depolarizing voltage commands evoked transient and sustained outward K-currents. The transient K-current was activated by depolarizing commands beyond -50 mV; peak current was dependent upon holding potential. Bath application of 4-aminopyridine, but not inorganic calcium channel blockers (Cd, Co, Mn), attenuated the transient current. Reversal was near the K-equilibrium potential. These properties suggest that this transient K-current is similar to the A-current described in a number of other neurons.

Original language	English (US)
Pages (from-to)	187-192
Number of pages	6
Journal	Brain research
Volume	473
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(88)90334-4
State	Published - Nov 8 1988

Keywords

A-current
Cell culture
Neostriatum
Voltage-clamp

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(88)90334-4

Cite this

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title = "Voltage-clamp analysis of a transient potassium current in rat neostriatal neurons",

abstract = "Whole cell voltage-clamp recording were made from cultured rat neostriatal neurons. Depolarizing voltage commands evoked transient and sustained outward K-currents. The transient K-current was activated by depolarizing commands beyond -50 mV; peak current was dependent upon holding potential. Bath application of 4-aminopyridine, but not inorganic calcium channel blockers (Cd, Co, Mn), attenuated the transient current. Reversal was near the K-equilibrium potential. These properties suggest that this transient K-current is similar to the A-current described in a number of other neurons.",

keywords = "A-current, Cell culture, Neostriatum, Voltage-clamp",

author = "Surmeier, {D. James} and Jose Bargas and Kitai, {S. T.}",

note = "Funding Information: We wish to thank Drs. V. Dionne, M. White and E. McCleskey for their help in bringing the patch clamp technique to our group. This work was supported by U.S.P.H.S. Grants NS20702 and NS23886.",

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doi = "10.1016/0006-8993(88)90334-4",

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AU - Surmeier, D. James

AU - Bargas, Jose

AU - Kitai, S. T.

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PY - 1988/11/8

Y1 - 1988/11/8

N2 - Whole cell voltage-clamp recording were made from cultured rat neostriatal neurons. Depolarizing voltage commands evoked transient and sustained outward K-currents. The transient K-current was activated by depolarizing commands beyond -50 mV; peak current was dependent upon holding potential. Bath application of 4-aminopyridine, but not inorganic calcium channel blockers (Cd, Co, Mn), attenuated the transient current. Reversal was near the K-equilibrium potential. These properties suggest that this transient K-current is similar to the A-current described in a number of other neurons.

AB - Whole cell voltage-clamp recording were made from cultured rat neostriatal neurons. Depolarizing voltage commands evoked transient and sustained outward K-currents. The transient K-current was activated by depolarizing commands beyond -50 mV; peak current was dependent upon holding potential. Bath application of 4-aminopyridine, but not inorganic calcium channel blockers (Cd, Co, Mn), attenuated the transient current. Reversal was near the K-equilibrium potential. These properties suggest that this transient K-current is similar to the A-current described in a number of other neurons.

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Voltage-clamp analysis of a transient potassium current in rat neostriatal neurons

Abstract

Keywords

ASJC Scopus subject areas

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