Voltage-dependent potassium currents during fast spikes of rat cerebellar Purkinje neurons: Inhibition by BDS-I toxin

Marco Martina*, Alexia E. Metz, Bruce P. Bean

*Corresponding author for this work

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We characterized the kinetics and pharmacological properties of voltage-activated potassium currents in rat cerebellar Purkinje neurons using recordings from nucleated patches, which allowed high resolution of activation and deactivation kinetics. Activation was exceptionally rapid, with 10-90% activation in about 400 μs at +30 mV, near the peak of the spike. Deactivation was also extremely rapid, with a decay time constant of about 300 μs near -80 mV. These rapid activation and deactivation kinetics are consistent with mediation by Kv3-family channels but are even faster than reported for Kv3-family channels in other neurons. The peptide toxin BDS-I had very little blocking effect on potassium currents elicited by 100-ms depolarizing steps, but the potassium current evoked by action potential waveforms was inhibited nearly completely. The mechanism of inhibition by BDS-I involves slowing of activation rather than total channel block, consistent with the effects described in cloned Kv3-family channels and this explains the dramatically different effects on currents evoked by short spikes versus voltage steps. As predicted from this mechanism, the effects of toxin on spike width were relatively modest (broadening by roughly 25%). These results show that BDS-I-sensitive channels with ultrafast activation and deactivation kinetics carry virtually all of the voltage-dependent potassium current underlying repolarization during normal Purkinje cell spikes.

Original languageEnglish (US)
Pages (from-to)563-571
Number of pages9
JournalJournal of neurophysiology
Volume97
Issue number1
DOIs
StatePublished - Jan 1 2007

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Purkinje Cells
Potassium
Evoked Potentials
Action Potentials
Pharmacology
Neurons
Peptides
scorpion toxin I''

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

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abstract = "We characterized the kinetics and pharmacological properties of voltage-activated potassium currents in rat cerebellar Purkinje neurons using recordings from nucleated patches, which allowed high resolution of activation and deactivation kinetics. Activation was exceptionally rapid, with 10-90{\%} activation in about 400 μs at +30 mV, near the peak of the spike. Deactivation was also extremely rapid, with a decay time constant of about 300 μs near -80 mV. These rapid activation and deactivation kinetics are consistent with mediation by Kv3-family channels but are even faster than reported for Kv3-family channels in other neurons. The peptide toxin BDS-I had very little blocking effect on potassium currents elicited by 100-ms depolarizing steps, but the potassium current evoked by action potential waveforms was inhibited nearly completely. The mechanism of inhibition by BDS-I involves slowing of activation rather than total channel block, consistent with the effects described in cloned Kv3-family channels and this explains the dramatically different effects on currents evoked by short spikes versus voltage steps. As predicted from this mechanism, the effects of toxin on spike width were relatively modest (broadening by roughly 25{\%}). These results show that BDS-I-sensitive channels with ultrafast activation and deactivation kinetics carry virtually all of the voltage-dependent potassium current underlying repolarization during normal Purkinje cell spikes.",
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Voltage-dependent potassium currents during fast spikes of rat cerebellar Purkinje neurons : Inhibition by BDS-I toxin. / Martina, Marco; Metz, Alexia E.; Bean, Bruce P.

In: Journal of neurophysiology, Vol. 97, No. 1, 01.01.2007, p. 563-571.

Research output: Contribution to journalArticle

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