Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the embryonic mouse skin and adult mouse cornea

Anees Fatima, Kathryn Marie-Schultz, Seungwoon Seo, Ford Culver, Austin Culver, Tsutomu Kume*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In order to study the lymphatic/blood vascular system, in mouse adult/embryonic tissues we often make use of histological sections. Although immunostaining of histologic sections does give an idea of the underlying vasculature, one of the shortcomings of these methods includes lack of possibilities of studying the vessel branching patterns. The whole mount staining of various mouse tissues provides an opportunity to study the entire vasculature including whole vessel width, vascular morphogenesis, and valve formations. Additionally data quantification of lymphatic/blood vasculature is often most accurate with whole mount immunostaining. In this chapter we present simple techniques for staining whole mount mouse embryonic dorsal skins (DS) and adult mouse corneal tissues for visualization of lymphatic and blood vasculature for research purposes.

Original languageEnglish (US)
Title of host publicationHandbook of Vascular Biology Techniques
PublisherSpringer Netherlands
Pages245-253
Number of pages9
ISBN (Electronic)9789401797160
ISBN (Print)9789401797153
DOIs
StatePublished - Jan 1 2015

Keywords

  • Blood vessel staining
  • Corneal flat mount
  • Embryo dorsal skin
  • Lymphatic vessel staining
  • Vascular patterning
  • Whole-mount immunostaining

ASJC Scopus subject areas

  • Medicine(all)
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Fatima, A., Marie-Schultz, K., Seo, S., Culver, F., Culver, A., & Kume, T. (2015). Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the embryonic mouse skin and adult mouse cornea. In Handbook of Vascular Biology Techniques (pp. 245-253). Springer Netherlands. https://doi.org/10.1007/978-94-017-9716-0_21