"Wide-open" 1.3 Å structure of a multidrug-resistant HIV-1 protease as a drug target

Philip Martin, John F. Vickrey, Gheorghe Proteasa, Yurytzy L. Jimenez, Zdzislaw Wawrzak, Mark A. Winters, Thomas C. Merigan, Ladislau C. Kovari*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    77 Scopus citations


    This report examines structural changes in a highly mutated, clinical multidrug-resistant HIV-1 protease, and the crystal structure has been solved to 1.3 Å resolution in the absence of any inhibitor. This protease variant contains codon mutations at positions 10, 36, 46, 54, 62, 63, 71, 82, 84, and 90 that confer resistance to protease inhibitors. Major differences between the wild-type and the variant include a structural change initiated by the M36V mutation and amplified by additional mutations in the flaps of the protease, resulting in a "wide-open" structure that represents an opening that is 8 Å wider than the "open" structure of the wild-type protease. A second structural change is triggered by the L90M mutation that results in reshaping the 23-32 segment. A third key structural change of the protease is due to the mutations from longer to shorter amino acid side chains at positions 82 and 84.

    Original languageEnglish (US)
    Pages (from-to)1887-1895
    Number of pages9
    Issue number12
    StatePublished - Dec 2005

    ASJC Scopus subject areas

    • Structural Biology
    • Molecular Biology

    Fingerprint Dive into the research topics of '"Wide-open" 1.3 Å structure of a multidrug-resistant HIV-1 protease as a drug target'. Together they form a unique fingerprint.

    Cite this