TY - JOUR
T1 - Wide variation in the cyanobacterial complement of presumptive penicillin-binding proteins
AU - Leganés, Francisco
AU - Blanco-Rivero, Amaya
AU - Fernández-Piñas, Francisca
AU - Redondo, Miguel
AU - Fernández-Valiente, Eduardo
AU - Fan, Qing
AU - Lechno-Yossef, Sigal
AU - Wolk, C. Peter
N1 - Funding Information:
Acknowledgments This work was supported by MCCYT grant BMC2003-03583, United States National Science Foundation grant MCB0090232, and United States Department of Energy grant DOE-FG02-91ER20021.
PY - 2005/12
Y1 - 2005/12
N2 - A genomic analysis of putative penicillin-binding proteins (PBPs) that are involved in the synthesis of the peptidoglycan layer of the cell wall and are encoded in 12 cyanobacterial genomes was performed in order to help elucidate the role(s) of these proteins in peptidoglycan synthesis, especially during cyanobacterial cellular differentiation. The analysis suggested that the minimum set of PBPs needed to assemble the peptidoglycan layer in cyanobacteria probably does not exceed one bifunctional transpeptidase-transglycosylase Class A high-molecular-weight PBP; two Class B high-molecular-weight PBPs, one of them probably involved in cellular elongation and the other in septum formation; and one low-molecular-weight PBP. The low-molecular-weight PBPs of all of the cyanobacteria analyzed are putative endopeptidases and are encoded by fewer genes than in Escherichia coli. We show that in Anabaena sp. strain PCC 7120, predicted proteins All2981 and Alr4579, like Alr5101, are Class A high-molecular-weight PBPs that are required for the functional differentiation of aerobically diazotrophic heterocysts, indicating that some members of this class of PBPs are required specifically for cellular developmental processes.
AB - A genomic analysis of putative penicillin-binding proteins (PBPs) that are involved in the synthesis of the peptidoglycan layer of the cell wall and are encoded in 12 cyanobacterial genomes was performed in order to help elucidate the role(s) of these proteins in peptidoglycan synthesis, especially during cyanobacterial cellular differentiation. The analysis suggested that the minimum set of PBPs needed to assemble the peptidoglycan layer in cyanobacteria probably does not exceed one bifunctional transpeptidase-transglycosylase Class A high-molecular-weight PBP; two Class B high-molecular-weight PBPs, one of them probably involved in cellular elongation and the other in septum formation; and one low-molecular-weight PBP. The low-molecular-weight PBPs of all of the cyanobacteria analyzed are putative endopeptidases and are encoded by fewer genes than in Escherichia coli. We show that in Anabaena sp. strain PCC 7120, predicted proteins All2981 and Alr4579, like Alr5101, are Class A high-molecular-weight PBPs that are required for the functional differentiation of aerobically diazotrophic heterocysts, indicating that some members of this class of PBPs are required specifically for cellular developmental processes.
KW - Cell differentiation
KW - Cell shape
KW - Cyanobacteria
KW - Fox genes
KW - Heterocysts
KW - Penicillin-binding protein
KW - Peptidoglycan
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U2 - 10.1007/s00203-005-0046-8
DO - 10.1007/s00203-005-0046-8
M3 - Article
C2 - 16231162
AN - SCOPUS:27744541317
SN - 0302-8933
VL - 184
SP - 234
EP - 248
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 4
ER -