Wild-type p53 binds to the TATA-binding protein and represses transcription

Edward Seto, Anny Usheva, Gerard P. Zambetti, Jamil Momand, Nobuo Horikoshi, Roberto Weinmann, Arnold J. Levine, Thomas Shenk

Research output: Contribution to journalArticle

451 Scopus citations

Abstract

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

Original languageEnglish (US)
Pages (from-to)12028-12032
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number24
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • General

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    Seto, E., Usheva, A., Zambetti, G. P., Momand, J., Horikoshi, N., Weinmann, R., Levine, A. J., & Shenk, T. (1992). Wild-type p53 binds to the TATA-binding protein and represses transcription. Proceedings of the National Academy of Sciences of the United States of America, 89(24), 12028-12032. https://doi.org/10.1073/pnas.89.24.12028