Wild-type p53 binds to the TATA-binding protein and represses transcription

Edward Seto; Anny Usheva; Gerard P. Zambetti; Jamil Momand; Nobuo Horikoshi; Roberto Weinmann; Arnold J. Levine; Thomas Shenk

Wild-type p53 binds to the TATA-binding protein and represses transcription

Edward Seto, Anny Usheva, Gerard P. Zambetti, Jamil Momand, Nobuo Horikoshi, Roberto Weinmann, Arnold J. Levine, Thomas Shenk

Radiation Oncology

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Abstract

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

Original language	English (US)
Pages (from-to)	12028-12032
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	24
State	Published - 1992

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title = "Wild-type p53 binds to the TATA-binding protein and represses transcription",

abstract = "p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.",

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year = "1992",

language = "English (US)",

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pages = "12028--12032",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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T1 - Wild-type p53 binds to the TATA-binding protein and represses transcription

AU - Seto, Edward

AU - Usheva, Anny

AU - Zambetti, Gerard P.

AU - Momand, Jamil

AU - Horikoshi, Nobuo

AU - Weinmann, Roberto

AU - Levine, Arnold J.

AU - Shenk, Thomas

PY - 1992

Y1 - 1992

N2 - p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

AB - p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

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SN - 0027-8424

VL - 89

SP - 12028

EP - 12032

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 24

ER -

Wild-type p53 binds to the TATA-binding protein and represses transcription

Abstract

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