Wilms' tumor 1 (WT1) gene in hematopoiesis: A surrogate marker of cell proliferation as a possible mechanism of action?

M. Olszewski, W. Huang, P. M. Chou, R. Duerst, Morris Kletzel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Background: Wilms' tumor 1 (WT1) gene expression is seen in a significant number of cases of human neoplasia; however the mechanism of action remains to be clarified. We hypothesized that WT1 gene is a surrogate marker of proliferation in normal hematopoietic cells and leukemias. While we and others have recognized its value as a tool for the detection of minimal residual disease (MRD), the objective of this study was to confirm our hypothesis regarding normal. Methods: Samples from healthy donors (n = 16) and UC blood (n = 9) were cultured in Methocult™ for 21 days. Colonies were analyzed on days 7, 14 and 21 by RT-PCR for WT1 gene expression. Our positive controls were ramples from patients with leukemia (n = 91). Negative controls were from normal volunteers without stimulation (n = 26). Results: Results showed a statistically significant difference (P < 0.0001) between cultured groups, with the highest level of WT1 gene expression in the positive controls and on day 14, when cells are at their maximal proliferation. Discussion: In conclusion, WT1 gene expression in the proliferating colonies was highest on day 14, although less than in leukemia samples, confirming our hypothesis that WT1 gene is a surrogate marker of proliferation, not only in leukemogenesis but also, to a lesser degree, in normal cell proliferation.

Original languageEnglish (US)
Pages (from-to)57-61
Number of pages5
JournalCytotherapy
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2005

Keywords

  • Hematopoiesis
  • Leukemia
  • Minimal residual disease
  • WT1 gene

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Cancer Research
  • Transplantation

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