Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics

Jae Woong Jeong; Jordan G. McCall; Gunchul Shin; Yihui Zhang; Ream Al-Hasani; Minku Kim; Shuo Li; Joo Yong Sim; Kyung In Jang; Yan Shi; Daniel Y. Hong; Yuhao Liu; Gavin P. Schmitz; Li Xia; Zhubin He; Paul Gamble; Wilson Z. Ray; Yonggang Huang; Michael R. Bruchas; John A. Rogers

doi:10.1016/j.cell.2015.06.058

Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics

Jae Woong Jeong, Jordan G. McCall, Gunchul Shin, Yihui Zhang, Ream Al-Hasani, Minku Kim, Shuo Li, Joo Yong Sim, Kyung In Jang, Yan Shi, Daniel Y. Hong, Yuhao Liu, Gavin P. Schmitz, Li Xia, Zhubin He, Paul Gamble, Wilson Z. Ray, Yonggang Huang, Michael R. Bruchas^*, John A. Rogers

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

386 Scopus citations

Abstract

In vivo pharmacology and optogenetics hold tremendous promise for dissection of neural circuits, cellular signaling, and manipulating neurophysiological systems in awake, behaving animals. Existing neural interface technologies, such as metal cannulas connected to external drug supplies for pharmacological infusions and tethered fiber optics for optogenetics, are not ideal for minimally invasive, untethered studies on freely behaving animals. Here, we introduce wireless optofluidic neural probes that combine ultrathin, soft microfluidic drug delivery with cellular-scale inorganic light-emitting diode (μ-ILED) arrays. These probes are orders of magnitude smaller than cannulas and allow wireless, programmed spatiotemporal control of fluid delivery and photostimulation. We demonstrate these devices in freely moving animals to modify gene expression, deliver peptide ligands, and provide concurrent photostimulation with antagonist drug delivery to manipulate mesoaccumbens reward-related behavior. The minimally invasive operation of these probes forecasts utility in other organ systems and species, with potential for broad application in biomedical science, engineering, and medicine.

Original language	English (US)
Pages (from-to)	662-674
Number of pages	13
Journal	Cell
Volume	162
Issue number	3
DOIs	https://doi.org/10.1016/j.cell.2015.06.058
State	Published - Aug 1 2015

Funding

This material is based on work supported by the EUREKA NIDA R01DA037152 (M.R.B.), NIMH F31 MH101956 (J.G.M.), and NIDA K99DA038725 (to R.A.). We thank the Bruchas laboratory and the laboratory of Dr. Robert W. Gereau IV, in particular Tayler Sheahan and Dr. Judith Golden (Washington University) for helpful discussion. We thank Dr. Karl Deisseroth (Stanford University) for the channelrhodopsin-2 (H134), Dr. Garret Stuber (UNC) for the TH-IRES-Cre mice, the WUSTL Hope Center Viral Vector Core for viral packaging, and the WUSTL Pain Center for use of the rotarod and running wheels. All biomedical aspects of the device work were supported by a National Security Science and Engineering Faculty Fellowship of Energy (J.A.R.). The LED development was enabled by funding from the US Department of Energy, Division of Materials Sciences under award number DE-FG02-07ER46471 (J.A.R.), the NIH Common Fund NINDS R01NS081707 (J.A.R. and M.R.B.), and through the Materials Research Laboratory and Center for Microanalysis of Materials ( DE-FG02-07ER46453 ) (J.A.R.).

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.cell.2015.06.058

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Jeong, J. W., McCall, J. G., Shin, G., Zhang, Y., Al-Hasani, R., Kim, M., Li, S., Sim, J. Y., Jang, K. I., Shi, Y., Hong, D. Y., Liu, Y., Schmitz, G. P., Xia, L., He, Z., Gamble, P., Ray, W. Z., Huang, Y., Bruchas, M. R., & Rogers, J. A. (2015). Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics. Cell, 162(3), 662-674. https://doi.org/10.1016/j.cell.2015.06.058

@article{344effb6c36a46348c220a3285475891,

title = "Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics",

abstract = "In vivo pharmacology and optogenetics hold tremendous promise for dissection of neural circuits, cellular signaling, and manipulating neurophysiological systems in awake, behaving animals. Existing neural interface technologies, such as metal cannulas connected to external drug supplies for pharmacological infusions and tethered fiber optics for optogenetics, are not ideal for minimally invasive, untethered studies on freely behaving animals. Here, we introduce wireless optofluidic neural probes that combine ultrathin, soft microfluidic drug delivery with cellular-scale inorganic light-emitting diode (μ-ILED) arrays. These probes are orders of magnitude smaller than cannulas and allow wireless, programmed spatiotemporal control of fluid delivery and photostimulation. We demonstrate these devices in freely moving animals to modify gene expression, deliver peptide ligands, and provide concurrent photostimulation with antagonist drug delivery to manipulate mesoaccumbens reward-related behavior. The minimally invasive operation of these probes forecasts utility in other organ systems and species, with potential for broad application in biomedical science, engineering, and medicine.",

author = "Jeong, {Jae Woong} and McCall, {Jordan G.} and Gunchul Shin and Yihui Zhang and Ream Al-Hasani and Minku Kim and Shuo Li and Sim, {Joo Yong} and Jang, {Kyung In} and Yan Shi and Hong, {Daniel Y.} and Yuhao Liu and Schmitz, {Gavin P.} and Li Xia and Zhubin He and Paul Gamble and Ray, {Wilson Z.} and Yonggang Huang and Bruchas, {Michael R.} and Rogers, {John A.}",

note = "Publisher Copyright: {\textcopyright}2015 Elsevier Inc.",

year = "2015",

month = aug,

day = "1",

doi = "10.1016/j.cell.2015.06.058",

language = "English (US)",

volume = "162",

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Jeong, JW, McCall, JG, Shin, G, Zhang, Y, Al-Hasani, R, Kim, M, Li, S, Sim, JY, Jang, KI, Shi, Y, Hong, DY, Liu, Y, Schmitz, GP, Xia, L, He, Z, Gamble, P, Ray, WZ, Huang, Y, Bruchas, MR & Rogers, JA 2015, 'Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics', Cell, vol. 162, no. 3, pp. 662-674. https://doi.org/10.1016/j.cell.2015.06.058

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AU - Jeong, Jae Woong

AU - McCall, Jordan G.

AU - Shin, Gunchul

AU - Zhang, Yihui

AU - Al-Hasani, Ream

AU - Kim, Minku

AU - Li, Shuo

AU - Sim, Joo Yong

AU - Jang, Kyung In

AU - Shi, Yan

AU - Hong, Daniel Y.

AU - Liu, Yuhao

AU - Schmitz, Gavin P.

AU - Xia, Li

AU - He, Zhubin

AU - Gamble, Paul

AU - Ray, Wilson Z.

AU - Huang, Yonggang

AU - Bruchas, Michael R.

AU - Rogers, John A.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - In vivo pharmacology and optogenetics hold tremendous promise for dissection of neural circuits, cellular signaling, and manipulating neurophysiological systems in awake, behaving animals. Existing neural interface technologies, such as metal cannulas connected to external drug supplies for pharmacological infusions and tethered fiber optics for optogenetics, are not ideal for minimally invasive, untethered studies on freely behaving animals. Here, we introduce wireless optofluidic neural probes that combine ultrathin, soft microfluidic drug delivery with cellular-scale inorganic light-emitting diode (μ-ILED) arrays. These probes are orders of magnitude smaller than cannulas and allow wireless, programmed spatiotemporal control of fluid delivery and photostimulation. We demonstrate these devices in freely moving animals to modify gene expression, deliver peptide ligands, and provide concurrent photostimulation with antagonist drug delivery to manipulate mesoaccumbens reward-related behavior. The minimally invasive operation of these probes forecasts utility in other organ systems and species, with potential for broad application in biomedical science, engineering, and medicine.

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Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics

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