Wnt ligand binding to and regulation of dopamine D2 receptors

Sehyoun Yoon; Mi Hyun Choi; Ja Hyun Baik

doi:10.1007/978-1-4939-2196-6_5

Wnt ligand binding to and regulation of dopamine D2 receptors

Sehyoun Yoon^*, Mi Hyun Choi, Ja Hyun Baik

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The dopamine D2 receptor (D2R) plays an important role in mesencephalic dopaminergic neuronal development, and we have previously reported that Wnt5a interacts with D2R to promote the differentiation of dopaminergic neurons. Co-immunoprecipitation of Wnt5a and D2R, displacement of the D2R receptor antagonist [ 3 H]spiperone from D2R by Wnt5a, as well as glutathione S-transferase (GST) pulldown assays demonstrated that Wnt5a binds D2R. In this chapter, we present the co-immunoprecipitation, pull-down, and competition-binding assays used in the analysis of the D2R-Wnt5a interaction.

Original language	English (US)
Title of host publication	Dopamine Receptor Technologies
Publisher	Springer New York
Pages	65-78
Number of pages	14
ISBN (Electronic)	9781493921966
ISBN (Print)	9781493921959
DOIs	https://doi.org/10.1007/978-1-4939-2196-6_5
State	Published - Dec 4 2014
Externally published	Yes

Keywords

Co-immunoprecipitation
Dopamine D2 receptor
GST pull-down assay
Radioligand binding
Wnt5a

ASJC Scopus subject areas

Medicine(all)
Neuroscience(all)

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10.1007/978-1-4939-2196-6_5

Cite this

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AU - Yoon, Sehyoun

AU - Choi, Mi Hyun

AU - Baik, Ja Hyun

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AB - The dopamine D2 receptor (D2R) plays an important role in mesencephalic dopaminergic neuronal development, and we have previously reported that Wnt5a interacts with D2R to promote the differentiation of dopaminergic neurons. Co-immunoprecipitation of Wnt5a and D2R, displacement of the D2R receptor antagonist [ 3 H]spiperone from D2R by Wnt5a, as well as glutathione S-transferase (GST) pulldown assays demonstrated that Wnt5a binds D2R. In this chapter, we present the co-immunoprecipitation, pull-down, and competition-binding assays used in the analysis of the D2R-Wnt5a interaction.

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KW - Dopamine D2 receptor

KW - GST pull-down assay

KW - Radioligand binding

KW - Wnt5a

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Wnt ligand binding to and regulation of dopamine D2 receptors

Abstract

Keywords

ASJC Scopus subject areas

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