Wnt ligand binding to and regulation of dopamine D2 receptors

Sehyoun Yoon; Mi Hyun Choi; Ja Hyun Baik

doi:10.1007/978-1-4939-2196-6_5

Wnt ligand binding to and regulation of dopamine D2 receptors

Sehyoun Yoon^*, Mi Hyun Choi, Ja Hyun Baik

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The dopamine D2 receptor (D2R) plays an important role in mesencephalic dopaminergic neuronal development, and we have previously reported that Wnt5a interacts with D2R to promote the differentiation of dopaminergic neurons. Co-immunoprecipitation of Wnt5a and D2R, displacement of the D2R receptor antagonist [³H]spiperone from D2R by Wnt5a, as well as glutathione S-transferase (GST) pulldown assays demonstrated that Wnt5a binds D2R. In this chapter, we present the co-immunoprecipitation, pull- down, and competition-binding assays used in the analysis of the D2R–Wnt5a interaction.

Original language	English (US)
Pages (from-to)	65-78
Number of pages	14
Journal	Neuromethods
Volume	96
DOIs	https://doi.org/10.1007/978-1-4939-2196-6_5
State	Published - 2015
Externally published	Yes

Keywords

Co-immunoprecipitation
Dopamine D2 receptor
GST pull-down assay
Radioligand binding
Wnt5a

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)
Psychiatry and Mental health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-2196-6_5

Cite this

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title = "Wnt ligand binding to and regulation of dopamine D2 receptors",

abstract = "The dopamine D2 receptor (D2R) plays an important role in mesencephalic dopaminergic neuronal development, and we have previously reported that Wnt5a interacts with D2R to promote the differentiation of dopaminergic neurons. Co-immunoprecipitation of Wnt5a and D2R, displacement of the D2R receptor antagonist [3H]spiperone from D2R by Wnt5a, as well as glutathione S-transferase (GST) pulldown assays demonstrated that Wnt5a binds D2R. In this chapter, we present the co-immunoprecipitation, pull- down, and competition-binding assays used in the analysis of the D2R–Wnt5a interaction.",

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year = "2015",

doi = "10.1007/978-1-4939-2196-6_5",

language = "English (US)",

volume = "96",

pages = "65--78",

journal = "Neuromethods",

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TY - JOUR

T1 - Wnt ligand binding to and regulation of dopamine D2 receptors

AU - Yoon, Sehyoun

AU - Choi, Mi Hyun

AU - Baik, Ja Hyun

PY - 2015

Y1 - 2015

N2 - The dopamine D2 receptor (D2R) plays an important role in mesencephalic dopaminergic neuronal development, and we have previously reported that Wnt5a interacts with D2R to promote the differentiation of dopaminergic neurons. Co-immunoprecipitation of Wnt5a and D2R, displacement of the D2R receptor antagonist [3H]spiperone from D2R by Wnt5a, as well as glutathione S-transferase (GST) pulldown assays demonstrated that Wnt5a binds D2R. In this chapter, we present the co-immunoprecipitation, pull- down, and competition-binding assays used in the analysis of the D2R–Wnt5a interaction.

AB - The dopamine D2 receptor (D2R) plays an important role in mesencephalic dopaminergic neuronal development, and we have previously reported that Wnt5a interacts with D2R to promote the differentiation of dopaminergic neurons. Co-immunoprecipitation of Wnt5a and D2R, displacement of the D2R receptor antagonist [3H]spiperone from D2R by Wnt5a, as well as glutathione S-transferase (GST) pulldown assays demonstrated that Wnt5a binds D2R. In this chapter, we present the co-immunoprecipitation, pull- down, and competition-binding assays used in the analysis of the D2R–Wnt5a interaction.

KW - Co-immunoprecipitation

KW - Dopamine D2 receptor

KW - GST pull-down assay

KW - Radioligand binding

KW - Wnt5a

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U2 - 10.1007/978-1-4939-2196-6_5

DO - 10.1007/978-1-4939-2196-6_5

M3 - Article

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SN - 0893-2336

VL - 96

SP - 65

EP - 78

JO - Neuromethods

JF - Neuromethods

ER -

Wnt ligand binding to and regulation of dopamine D2 receptors

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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