Wnt signaling though beta-catenin is required for prostate lineage specification

Brian W. Simons; Paula J. Hurley; Zhenhua Huang; Ashley E. Ross; Rebecca Miller; Luigi Marchionni; David M. Berman; Edward M. Schaeffer

doi:10.1016/j.ydbio.2012.08.016

Wnt signaling though beta-catenin is required for prostate lineage specification

Brian W. Simons^*, Paula J. Hurley, Zhenhua Huang, Ashley E. Ross, Rebecca Miller, Luigi Marchionni, David M. Berman, Edward M. Schaeffer

^*Corresponding author for this work

Urology

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Androgens initiate a complex network of signals within the UGS that trigger prostate lineage commitment and bud formation. Given its contributions to organogenesis in other systems, we investigated a role for canonical Wnt signaling in prostate development. We developed a new method to achieve complete deletion of beta-catenin, the transcriptional coactivator required for canonical Wnt signaling, in early prostate development. Beta-catenin deletion abrogated canonical Wnt signaling and yielded prostate rudiments that exhibited dramatically decreased budding and failed to adopt prostatic identity. This requirement for canonical Wnt signaling was limited to a brief critical period during the initial molecular phase of prostate identity specification. Deletion of beta-catenin in the adult prostate did not significantly affect organ homeostasis. Collectively, these data establish that beta-catenin and Wnt signaling play key roles in prostate lineage specification and bud outgrowth.

Original language	English (US)
Pages (from-to)	246-255
Number of pages	10
Journal	Developmental Biology
Volume	371
Issue number	2
DOIs	https://doi.org/10.1016/j.ydbio.2012.08.016
State	Published - Nov 15 2012

Funding

The authors thank Dr. Michael Shen, University of Columbia , for providing Nkx3.1-Cre mice. BWS was supported by NIH T32 RR007002 and DOD PC080778 . LM was supported by P30CA006973 . EMS was supported by NIH DK081019 . EMS is an AUA Astellas Rising Star and HHMI Early Careers Physician Scientist. DMB was supported by NIH 5R01DK72000-5.

Keywords

Mouse
Prostate development
Urogenital sinus
Wnt signaling

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.ydbio.2012.08.016

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title = "Wnt signaling though beta-catenin is required for prostate lineage specification",

abstract = "Androgens initiate a complex network of signals within the UGS that trigger prostate lineage commitment and bud formation. Given its contributions to organogenesis in other systems, we investigated a role for canonical Wnt signaling in prostate development. We developed a new method to achieve complete deletion of beta-catenin, the transcriptional coactivator required for canonical Wnt signaling, in early prostate development. Beta-catenin deletion abrogated canonical Wnt signaling and yielded prostate rudiments that exhibited dramatically decreased budding and failed to adopt prostatic identity. This requirement for canonical Wnt signaling was limited to a brief critical period during the initial molecular phase of prostate identity specification. Deletion of beta-catenin in the adult prostate did not significantly affect organ homeostasis. Collectively, these data establish that beta-catenin and Wnt signaling play key roles in prostate lineage specification and bud outgrowth.",

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note = "Funding Information: The authors thank Dr. Michael Shen, University of Columbia , for providing Nkx3.1-Cre mice. BWS was supported by NIH T32 RR007002 and DOD PC080778 . LM was supported by P30CA006973 . EMS was supported by NIH DK081019 . EMS is an AUA Astellas Rising Star and HHMI Early Careers Physician Scientist. DMB was supported by NIH 5R01DK72000-5. ",

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doi = "10.1016/j.ydbio.2012.08.016",

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AU - Simons, Brian W.

AU - Hurley, Paula J.

AU - Huang, Zhenhua

AU - Ross, Ashley E.

AU - Miller, Rebecca

AU - Marchionni, Luigi

AU - Berman, David M.

AU - Schaeffer, Edward M.

N1 - Funding Information: The authors thank Dr. Michael Shen, University of Columbia , for providing Nkx3.1-Cre mice. BWS was supported by NIH T32 RR007002 and DOD PC080778 . LM was supported by P30CA006973 . EMS was supported by NIH DK081019 . EMS is an AUA Astellas Rising Star and HHMI Early Careers Physician Scientist. DMB was supported by NIH 5R01DK72000-5.

PY - 2012/11/15

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N2 - Androgens initiate a complex network of signals within the UGS that trigger prostate lineage commitment and bud formation. Given its contributions to organogenesis in other systems, we investigated a role for canonical Wnt signaling in prostate development. We developed a new method to achieve complete deletion of beta-catenin, the transcriptional coactivator required for canonical Wnt signaling, in early prostate development. Beta-catenin deletion abrogated canonical Wnt signaling and yielded prostate rudiments that exhibited dramatically decreased budding and failed to adopt prostatic identity. This requirement for canonical Wnt signaling was limited to a brief critical period during the initial molecular phase of prostate identity specification. Deletion of beta-catenin in the adult prostate did not significantly affect organ homeostasis. Collectively, these data establish that beta-catenin and Wnt signaling play key roles in prostate lineage specification and bud outgrowth.

AB - Androgens initiate a complex network of signals within the UGS that trigger prostate lineage commitment and bud formation. Given its contributions to organogenesis in other systems, we investigated a role for canonical Wnt signaling in prostate development. We developed a new method to achieve complete deletion of beta-catenin, the transcriptional coactivator required for canonical Wnt signaling, in early prostate development. Beta-catenin deletion abrogated canonical Wnt signaling and yielded prostate rudiments that exhibited dramatically decreased budding and failed to adopt prostatic identity. This requirement for canonical Wnt signaling was limited to a brief critical period during the initial molecular phase of prostate identity specification. Deletion of beta-catenin in the adult prostate did not significantly affect organ homeostasis. Collectively, these data establish that beta-catenin and Wnt signaling play key roles in prostate lineage specification and bud outgrowth.

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KW - Urogenital sinus

KW - Wnt signaling

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Wnt signaling though beta-catenin is required for prostate lineage specification

Abstract

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Keywords

ASJC Scopus subject areas

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