Abstract
We have determined single crystal structures of an A-DNA decamer and a B-DNA dodecamer at 0.83 and 0.95 Å, respectively. The resolution of the former is the highest reported thus far for any right-handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A- and B-form DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition. (C) 2000 John Wiley and Sons, Inc.
Original language | English (US) |
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Pages (from-to) | 234-252 |
Number of pages | 19 |
Journal | Biopolymers |
Volume | 48 |
Issue number | 4 |
DOIs | |
State | Published - 1998 |
Funding
Keywords
- A-DNA
- B-DNA
- Direct methods
- Hydration
- Structural transition
- Water structure
- X-ray crystallography
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- Biochemistry
- Biophysics
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Dive into the research topics of 'X-ray crystallographic analysis of the hydration of A- and B-form DNA at atomic resolution'. Together they form a unique fingerprint.Datasets
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B-DODECAMER CGCGAA(TAF)TCGCG WITH INCORPORATED 2'-DEOXY-2'-FLUORO-ARABINO-FURANOSYL THYMINE
Egli, M. (Contributor), Tereshko, V. (Contributor), Teplova, M. (Contributor), Minasov, G. (Contributor), Joachimiak, A. (Contributor), Sanishvili, R. (Contributor), Weeks, C. M. (Contributor), Miller, R. (Contributor), Maier, M. A. (Contributor), An, H. (Contributor), Dan, C. P. (Contributor) & Manoharan, M. (Contributor), Protein Data Bank (PDB), Apr 4 2000
DOI: 10.2210/pdb1DPN/pdb, https://www.wwpdb.org/pdb?id=pdb_00001dpn
Dataset
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A-DNA DECAMER GCGTA(T23)TACGC WITH INCORPORATED 2'-METHOXY-3'-METHYLENEPHOSPHATE-THYMIDINE
Egli, M. (Contributor), Tereshko, V. (Contributor), Teplova, M. (Contributor), Minasov, G. (Contributor), Joachimiak, A. (Contributor), Sanishvili, R. (Contributor), Weeks, C. M. (Contributor), Miller, R. (Contributor), Maier, M. A. (Contributor), An, H. (Contributor), Dan, C. P. (Contributor) & Manoharan, M. (Contributor), Protein Data Bank (PDB), Apr 4 2000
DOI: 10.2210/pdb1DPL/pdb, https://www.wwpdb.org/pdb?id=pdb_00001dpl
Dataset