X-ray crystallographic analysis of the hydration of A- and B-form DNA at atomic resolution

Martin Egli*, Valentina Tereshko, Marianna Teplova, George Minasov, Andrzei Joachimiak, Ruslan Sanishvili, Charles M. Weeks, Russ Miller, Martin A. Maier, Haoyun An, P. Dan Cook, Muthiah Manoharan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

123 Scopus citations

Abstract

We have determined single crystal structures of an A-DNA decamer and a B-DNA dodecamer at 0.83 and 0.95 Å, respectively. The resolution of the former is the highest reported thus far for any right-handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A- and B-form DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)234-252
Number of pages19
JournalBiopolymers
Volume48
Issue number4
DOIs
StatePublished - 1998

Keywords

  • A-DNA
  • B-DNA
  • Direct methods
  • Hydration
  • Structural transition
  • Water structure
  • X-ray crystallography

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

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