X-ray nanoprobes and diffraction-limited storage rings: Opportunities and challenges of fluorescence tomography of biological specimens

Martin D. De Jonge, Christopher G. Ryan, Chris J. Jacobsen

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

X-ray nanoprobes require coherent illumination to achieve optic-limited resolution, and so will benefit directly from diffraction-limited storage rings. Here, the example of high-resolution X-ray fluorescence tomography is focused on as one of the most voracious demanders of coherent photons, since the detected signal is only a small fraction of the incident flux. Alternative schemes are considered for beam delivery, sample scanning and detectors. One must consider as well the steps before and after the X-ray experiment: sample preparation and examination conditions, and analysis complexity due to minimum dose requirements and self-absorption. By understanding the requirements and opportunities for nanoscale fluorescence tomography, one gains insight into the R&D challenges in optics and instrumentation needed to fully exploit the source advances that diffraction-limited storage rings offer.

Original languageEnglish (US)
Pages (from-to)1031-1047
Number of pages17
JournalJournal of Synchrotron Radiation
Volume21
Issue number5
DOIs
StatePublished - Sep 2014

Keywords

  • X-ray fluorescence microscopy
  • diffraction-limited storage rings
  • fluorescence tomography
  • nanotomography
  • scanning X-ray microscopy

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

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