X-ray solution structure of the native neuronal porosome-synaptic vesicle complex: Implication in neurotransmitter release

Ladislau C. Kovari, Joseph S. Brunzelle, Kenneth T. Lewis, Won Jin Cho, Jin Sook Lee, Douglas J. Taatjes, Bhanu P. Jena*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Nanoportals at the cell plasma membrane called porosomes, mediate secretion from cells. In neurons porosomes are 15. nm cup-shaped lipoprotein structure composed of nearly 40 proteins. The size and complexity of the porosome has precluded determination of its atomic structure. Here we report at nanometer resolution the native 3D structure of the neuronal porosome-synaptic vesicle complex within isolated nerve terminals using small-angle X-ray solution scattering. In addition to furthering our understanding of the porosome structure, results from the study suggests the molecular mechanism involved in neurotransmitter release at the nerve terminal.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalMicron
Volume56
DOIs
StatePublished - Jan 2014

Keywords

  • Electron microscopy
  • Neuronal porosome complex
  • Photon correlation spectroscopy
  • X-ray solution scattering

ASJC Scopus subject areas

  • Structural Biology
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Cell Biology

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