@article{471a951355e042d993b9e6f201774e8e,
title = "X-ray solution structure of the native neuronal porosome-synaptic vesicle complex: Implication in neurotransmitter release",
abstract = "Nanoportals at the cell plasma membrane called porosomes, mediate secretion from cells. In neurons porosomes are 15. nm cup-shaped lipoprotein structure composed of nearly 40 proteins. The size and complexity of the porosome has precluded determination of its atomic structure. Here we report at nanometer resolution the native 3D structure of the neuronal porosome-synaptic vesicle complex within isolated nerve terminals using small-angle X-ray solution scattering. In addition to furthering our understanding of the porosome structure, results from the study suggests the molecular mechanism involved in neurotransmitter release at the nerve terminal.",
keywords = "Electron microscopy, Neuronal porosome complex, Photon correlation spectroscopy, X-ray solution scattering",
author = "Kovari, {Ladislau C.} and Brunzelle, {Joseph S.} and Lewis, {Kenneth T.} and Cho, {Won Jin} and Lee, {Jin Sook} and Taatjes, {Douglas J.} and Jena, {Bhanu P.}",
note = "Funding Information: Supported by grants from NIH NS-39918 and Wayne State University Research Enhancement Award (BPJ) . We are grateful to Steven Weigand for technical assistance and help with data collection at DND-CAT. The DuPont-Northwestern-Dow Collaborative Access Team (DND-CAT, sector 5) Synchrotron Research Center at the Advanced Photon Source (APS, Argonne, IL) is supported by E. I. DuPont de Nemours & Co., The Dow Chemical Company, the National Science Foundation, and the State of Illinois . Use of the Advanced Photon Source, an Office of Science User Facility operated for the U.S. Department of Energy (DOE) Office of Science by Argonne National Laboratory, was supported by the U.S. DOE under Contract No. DE-AC02-06CH11357 . Contributions: BPJ designed the research and wrote the paper. JSB and LCK performed the SAXS and associated calculation and analysis of the data. KTL isolated synaptosomes, performed PCS on them, and helped in preparation of the micrographs for the manuscript. DJT performed the EM studies on the isolated synaptosomes. WJC and JSL prepared synaptosomes, helped in drawing the cartoon, and in the preparation of the micrographs. BPJ, DJT, JSB and LCK critically analyzed results and proof read the manuscript.",
year = "2014",
month = jan,
doi = "10.1016/j.micron.2013.10.002",
language = "English (US)",
volume = "56",
pages = "37--43",
journal = "Micron",
issn = "0968-4328",
publisher = "Elsevier Limited",
}