Yeast TFIIE: Cloning, expression, and homology to vertebrate proteins

W. J. Feaver; N. L. Henry; D. A. Bushnell; M. H. Sayre; J. H. Brickner; O. Gileadi; R. D. Kornberg

Yeast TFIIE: Cloning, expression, and homology to vertebrate proteins

W. J. Feaver, N. L. Henry, D. A. Bushnell, M. H. Sayre, J. H. Brickner, O. Gileadi, R. D. Kornberg^*

^*Corresponding author for this work

Molecular Biosciences

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52 Scopus citations

Abstract

Genes encoding both the 66- and the 43-kDa subunits of yeast RNA polymerase II initiation factor a, designated TFA1 and TFA2, have been isolated. Both genes are essential for cell viability. The bacterially expressed gene products could replace factor a in transcription in vitro, and both recombinant subunits were required for activity. The deduced amino acid sequences of the TFA1 and TFA2 gene products were homologous to those of the large and small subunits of human TFIIE, respectively, identifying factor a as the yeast homolog of TFIIE.

Original language	English (US)
Pages (from-to)	27549-27553
Number of pages	5
Journal	Journal of Biological Chemistry
Volume	269
Issue number	44
State	Published - Nov 4 1994

Funding

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

Cite this

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title = "Yeast TFIIE: Cloning, expression, and homology to vertebrate proteins",

abstract = "Genes encoding both the 66- and the 43-kDa subunits of yeast RNA polymerase II initiation factor a, designated TFA1 and TFA2, have been isolated. Both genes are essential for cell viability. The bacterially expressed gene products could replace factor a in transcription in vitro, and both recombinant subunits were required for activity. The deduced amino acid sequences of the TFA1 and TFA2 gene products were homologous to those of the large and small subunits of human TFIIE, respectively, identifying factor a as the yeast homolog of TFIIE.",

author = "Feaver, {W. J.} and Henry, {N. L.} and Bushnell, {D. A.} and Sayre, {M. H.} and Brickner, {J. H.} and O. Gileadi and Kornberg, {R. D.}",

year = "1994",

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day = "4",

language = "English (US)",

volume = "269",

pages = "27549--27553",

journal = "Journal of Biological Chemistry",

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TY - JOUR

T1 - Yeast TFIIE

T2 - Cloning, expression, and homology to vertebrate proteins

AU - Feaver, W. J.

AU - Henry, N. L.

AU - Bushnell, D. A.

AU - Sayre, M. H.

AU - Brickner, J. H.

AU - Gileadi, O.

AU - Kornberg, R. D.

PY - 1994/11/4

Y1 - 1994/11/4

N2 - Genes encoding both the 66- and the 43-kDa subunits of yeast RNA polymerase II initiation factor a, designated TFA1 and TFA2, have been isolated. Both genes are essential for cell viability. The bacterially expressed gene products could replace factor a in transcription in vitro, and both recombinant subunits were required for activity. The deduced amino acid sequences of the TFA1 and TFA2 gene products were homologous to those of the large and small subunits of human TFIIE, respectively, identifying factor a as the yeast homolog of TFIIE.

AB - Genes encoding both the 66- and the 43-kDa subunits of yeast RNA polymerase II initiation factor a, designated TFA1 and TFA2, have been isolated. Both genes are essential for cell viability. The bacterially expressed gene products could replace factor a in transcription in vitro, and both recombinant subunits were required for activity. The deduced amino acid sequences of the TFA1 and TFA2 gene products were homologous to those of the large and small subunits of human TFIIE, respectively, identifying factor a as the yeast homolog of TFIIE.

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M3 - Article

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SN - 0021-9258

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 44

ER -

Yeast TFIIE: Cloning, expression, and homology to vertebrate proteins

Abstract

Funding

ASJC Scopus subject areas

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