Zinc sparks are triggered by fertilization and facilitate cell cycle resumption in mammalian eggs

Alison M. Kim, Miranda L. Bernhardt, Betty Y. Kong, Richard W. Ahn, Stefan Vogt, Teresa K. Woodruff*, Thomas V. O'Halloran

*Corresponding author for this work

Research output: Contribution to journalArticle

116 Scopus citations

Abstract

In last few hours of maturation, the mouse oocyte takes up over twenty billion zinc atoms and arrests after the first meiotic division, until fertilization or pharmacological intervention stimulates cell cycle progression toward a new embryo. Using chemical and physical probes, we show that fertilization of the mature, zinc-enriched egg triggers the ejection of zinc into the extracellular milieu in a series of coordinated events termed zinc sparks. These events immediately follow the well-established series of calcium oscillations within the activated egg and are evolutionarily conserved in several mammalian species, including rodents and nonhuman primates. Functionally, the zinc sparks mediate a decrease in intracellular zinc content that is necessary for continued cell cycle progression, as increasing zinc levels within the activated egg results in the reestablishment of cell cycle arrest at metaphase. The mammalian egg thus uses a zinc-dependent switch mechanism to toggle between metaphase arrest and resumption of the meiotic cell cycle at the initiation of embryonic development.

Original languageEnglish (US)
Pages (from-to)716-723
Number of pages8
JournalACS chemical biology
Volume6
Issue number7
DOIs
StatePublished - Jul 15 2011

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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