TY - JOUR
T1 - Zwittermicin A biosynthetic cluster
AU - Stohl, Elizabeth A.
AU - Milner, Jocelyn L.
AU - Handelsman, Jo
N1 - Funding Information:
This work was supported by the University of Wisconsin-Madison University-Industry Research Program, Hatch Project 4038, and the University of Wisconsin-Madison College of Agricultural and Life Sciences.
PY - 1999/9/17
Y1 - 1999/9/17
N2 - The goal of this study was to identify the biosynthetic cluster for zwittermicin A, a novel, broad spectrum, aminopolyol antibiotic produced by Bacillus cereus. The nucleotide sequence of 2.7 kb of DNA flanking the zwittermicin A self-resistance gene, zmaR, from B. cereus UW85 revealed three open reading frames (ORFs). Of these ORFs, two had sequence similarity to acyl-CoA dehydrogenases and polyketide synthases, respectively. Insertional inactivation demonstrated that orf2 is necessary for zwittermicin A production and that zmaR is necessary for high-level resistance to zwittermicin A but is not required for zwittermicin A production. Expression of ZmaR was temporally associated with zwittermicin A production. The results suggest that zmaR is part of a cluster of genes that is involved in zwittermicin A biosynthesis, representing the first biosynthetic pathway for an aminopolyol antibiotic.
AB - The goal of this study was to identify the biosynthetic cluster for zwittermicin A, a novel, broad spectrum, aminopolyol antibiotic produced by Bacillus cereus. The nucleotide sequence of 2.7 kb of DNA flanking the zwittermicin A self-resistance gene, zmaR, from B. cereus UW85 revealed three open reading frames (ORFs). Of these ORFs, two had sequence similarity to acyl-CoA dehydrogenases and polyketide synthases, respectively. Insertional inactivation demonstrated that orf2 is necessary for zwittermicin A production and that zmaR is necessary for high-level resistance to zwittermicin A but is not required for zwittermicin A production. Expression of ZmaR was temporally associated with zwittermicin A production. The results suggest that zmaR is part of a cluster of genes that is involved in zwittermicin A biosynthesis, representing the first biosynthetic pathway for an aminopolyol antibiotic.
KW - Acyl-CoA dehydrogenases
KW - Acyltransferases
KW - Polyketide synthases
KW - Self-resistance
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U2 - 10.1016/S0378-1119(99)00315-7
DO - 10.1016/S0378-1119(99)00315-7
M3 - Article
C2 - 10521664
AN - SCOPUS:0032861623
SN - 0378-1119
VL - 237
SP - 403
EP - 411
JO - Gene
JF - Gene
IS - 2
ER -